朱敦明

中国科学院天津工业生物技术研究所

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  • 朱敦明
  • 研究员
  • 天津空港经济区西七道32号

简历

2012年11月至2014年4月 中国科学院天津工业生物技术研究所副所长

2008年至今 中国科学院天津工业生物技术所研究员,博士生导师,工业酶国家工程实验室副主任,

2004年至今 Research Professor, Department of Chemistry, Southern Methodist University, Dallas, TX 75275, USA

2000年至2004年 Staff Scientist and Group Leader, BioCatalytics, Inc., Pasadena, CA, USA

1996年至1999年 Research Associate, Professor J. K. Kochi, University of Houston, Houston, TX, USA

1994年至1996年 Postdoctoral Associate, Professor N. S. Hosmane, Southern Methodist University, Dallas, Texas, USA

1993年至1994年 助理研究员,中国科学院上海有机化学研究所

现任生物工程学报编委会成员、中国微生物学会酶工程专业委员会委员、中国生物工程学会氨基酸专业委员会委员、中国药学会制药工程专业委员会委员。

在Nature Catalysis, JACS,ASCCS Catalysis,Green Chemistry, Org. Lett,Advanced Synthesis and Catalysis, Applied and Environmental MicrobiologyEM等刊物发表论文100多篇,邀请综述和专著章节10篇;申请专利30多项,美国专利授权1项、发明专利授权6项。

研究方向:

合成生物学与合成化学交叉领域,生物催化与绿色化工

代表论著:

1. X. Chen, H. Zhang, M. A. Maria-Solano, W. Liu, J. Li, J. Feng, X. Liu, S. Osuna, R.-T. Guo, Q. Wu, D. Zhu, Y. Ma, “Efficient Reductive Desymmetrization of Bulky Prochiral 1,3-Cyclodiketone Enabled by Structure-Guided Directed Evolution of a Carbonyl Reductase”, Nature Catalysis, 2019, doi: 10.1038/s41929-019-0347-y.

2. N. Liu, J. Feng, R. Zhang, X. Chen, X. Li, P. Yao, Q. Wu, Y. Ma, D. Zhu, “Efficient Microbial Synthesis of Key Steroidal Intermediates from Bio-Renewable Phytosterols by Genetically Modified Mycobacterium fortuitum Strains”, Green Chemistry, 2019, 21, 4076–4083. https://doi.org/10.1039/c9gc01267b.

3. Q. Chen, X. Chen, J. Feng, Q. Wu, D. Zhu, Y. Ma “Improving and inverting Cb-stereoselectivity of threonine aldolase via substrate-binding-guided mutagenesis and a stepwise visual screening”, ACS Catalysis, 2019, 9, 4462-4469. https://doi.org/10.1021/acscatal.9b00859.

4. S. Yu, P. Yao, J. Li, J. Feng, Q. Wu, D. Zhu, “Improving catalytic efficiency and stereoselectivity of a nitrilase from Syechocystis sp. PCC6803 by semi-rational engineering en route to chiral g-amino acids”, Catalysis Science and Technology, 2019, 9, 1504-1510. https://doi.org/10.1039/C8CY02455C (selected as Hot Article).

5. P. Yao, Z. Xu, S. Yu, Q. Wu, D. Zhu, “Imine Reductase-Catalyzed Enantioselective Reduction of Bulky α, β-Unsaturated Imines en Route to a Pharmaceutically Important Morphinan Skeleton”, Advanced Synthesis and Catalysis, 2019, 361, 556-561. https://doi.org/10.1002/adsc.201801326 (selected as “Hot Topic: Biocatalysis” article).

6. W. Lu, X. Chen, Y. Wang, J. Feng, Q. Wu, D. Zhu, “A Fungal P450 from Thanatephorus cucumeris with Novel Steroid Hydroxylation Capabilities”, Applied and Environmental Microbiology, 2018, 84:e00503-18. DOI: 10.1128/AEM.00503-18 (selected as Spotlight Article).

7. Y. Zhang, P. Yao, Y. Cui, Q. Wu, D. Zhu, “One-pot enzymatic synthesis of cyclic vicinal diols from aliphatic dialdehydes via intramolecular C?C bond formation and carbonyl reduction”, Advanced Synthesis and Catalysis, 2018, 360, 4191-4196. DOI: 10.1002/adsc.201800455.

8. X. Chen, H. Zhang, J. Feng, Q. Wu, D. Zhu, “Molecular basis for the high activity and enantioselectivity of carbonyl reductase from Sporobolomyces salmonicolor toward α-halo-acetophenones”, ACS Catalysis, 2018, 8, 3525-3531, DOI: 10.1021/acscatal.8b00591.

9. P. Yao, P. Cong, R. Gong, J. Li, G. Li, J. Ren, J. Feng, J. Lin, P. C. K. Lau, Q. Wu, D. Zhu, “Biocatalytic Route to Chiral 2-Substituted-1, 2, 3, 4-tetrahydroquinolines Using Cyclohexylamine Oxidase Muteins”, ACS Catalysis, 2018, 8, 1648-1652, DOI: 10.1021/acscatal.7b03552.

10. Q. Chen,X. Chen, Y. Cui, J. Ren,W. Lu, J Feng, Q. Wu, D. Zhu, “A new D-threonine aldolase as promising biocatalyst for highly stereoselective preparation of chiral aromatic β-hydroxy-α-amino acids”, Catalysis Science and Technology, 2017, 7, 5964-5973. DOI/ 10.1039/C7CY01774J.

11. G. Li, P. Yao, R. Gong, J. Li, P. Liu, R. Lonsdale, Q. Wu, J. Lin, D. Zhu, M. T. Reetz, “Simultaneous engineering of an enzyme’s entrance tunnel and active site: The case of monoamine oxidase MAO-N”, Chemical Science, 2017, 8, 4093-4099.

12. P. Yao, Y. Cui, S. Yu, Y. Du, J. Feng, Q. Wu, D. Zhu, “Efficient biosynthesis of (R)- or (S)-2-hydroxybutyrate from L-threonine through a synthetic biology approach”, Advanced Synthesis and Catalysis, 2016, 358, 2923-2928.

13. J. Ren, P. Yao, S. Yu, W. Dong, Q. Chen, J. Feng, Q. Wu, D. Zhu, “An Unprecedented Effective Enzymatic Carboxylation of Phenols”, ACS Catalysis, 2016, 5, 564-567 (X-MOL平台报道).

14. D. Zhang, X. Chen, J. Chi, J. Feng, Q. Wu, D. Zhu, “Semi–Rational Engineering a Carbonyl Reductase to Enhance the Enzyme Activity and Enantioselectivity for the Reduction of b-Amino Ketones”, ACS Catalysis, 2015, 5, 2452-2457.

15. P. Yao, L. Wang, J. Yuan, L. Cheng, R. Jia, M. Xie, J. Feng, M. Wang, Q. Wu, D. Zhu, “Novel Biosynthesis of Ethyl (R)-3-Hydroxyglutarate through Halohydrin Dehalogenase and Nitrilase Bienzymatic Cascade”, ChemCatChem, 2015, 7, 1438-1444 (Featured in the cover).

16. D. Zhang, X. Chen, R. Zhang, P. Yao, Q. Wu, D. Zhu, “Development of β-Amino Acid Dehydrogenase for the Synthesis of β-Amino Acids via Reductive Amination of β-Keto Acids”, ACS Catalysis, 2015, 5, 2220-2224.

17. Y. Duan, P. Yao, J. Ren, C. Han, Q. Li, J. Yuan, J. Feng, Q. Wu, D. Zhu, “Biocatalytic desymmetrization of 3-substituted glutaronitriles by nitrilases. A convenient chemoenzymatic access to optically active (S)-Pregabalin and (R)-Baclofen”, SCIENCE CHINA Chemistry, 2014, 57, 1164-1171.

18. G. Li, J. Ren, P. Yao, Y. Duan, H. Zhang, Q. Wu, J. Feng, P. C. K. Lau, D. Zhu, “Deracemization of 2-Methyl-1, 2, 3, 4-tetrahydroquinoline Using Mutant Cyclohexylamine Oxidase Obtained by Iterative Saturation Mutagenesis”, ACS Catalysis, 2014, 4, 903-908 (Featured in the cover).

19. X. Gao, X. Chen, W. Liu, J. Feng, Q. Wu, L. Hua, D. Zhu, “A Novel meso-Diaminopimelate Dehydrogenase from Symbiobacterium thermophilum. Overexpression, Characterizationand Potential for D-amino Acid Synthesis”, Applied and Environmental Microbiology, 2012, 78, 8595-8600.

20. D. Zhu, Y. Yang, S. Majkowitcz, T. H.-Y. Pan, K. Kantardjieff, L. Hua, “Inverting the Enantioselectivity of a Carbonyl Reductase via Enzyme-Substrate Docking-Guided Point Mutation”, Organic Letters, 2008, 10, 525-528.

21. D. Zhu, H. Ankati, C. Mukherjee, Y. Yang, E. R. Biehl, L. Hua, “Asymmetric Reduction of b-Ketonitriles with a Recombinant Carbonyl Reductase and Enzymatic Transformation to Optically Pure b-Hydroxy Carboxylic Acids”, Organic Letters, 2007, 9, 2561-2563 (Highlighted in Synfacts 2007, 8, 0876, Organic Chemistry Portal and Identified as an “Article of Interest to the Pharmaceutical Industry” by ACS GCI Pharmaceutical Roundtable (Organic Process Research & Development, 2008, 12(5))).

22. N. S. Hosmane, D. Zhu, J. E. McDonald, H. Zhang, J. A. Maguire, T. G. Gray, S. C. Helfert, “Magnesium Alkyls As Metallating Reagents in Formation of Novel Half- and Full-Sandwich Magnesacarboranes”, J. Am. Chem. Soc., 1995, 117, 12362.

承担科研项目情况:

2018.01-2021.12,国家自然科学基金面上项目,21778072,β-氨基酸脱氢酶多样性、底物识别及立体控制机理的研究;

2015.01-2018.12,国家自然科学基金面上项目,21472232,芳香羧酸脱羧酶催化C-H键活化及二氧化碳固定化的研究;

2011.01-2013.12,国家自然科学基金面上项目,21072151,生物催化不对称羰基还原胺化反应的探索;

2011.01-2015.10,国家重点基础研究发展计划“973”项目“生物制造手性化学品的科学基础”之课题1“生物合成的手性识别机理解析”, 2011CB710801;

2009年-2012年, 中国科学院支持天津滨海新区建设科技行动计划项目“发酵法生产谷氨酰胺转胺酶的中试研究”;

2009年-2012年 , 中国科学院知识创新工程重要方向项目“L-天冬氨酸的绿色生产新工艺”之子课题“顺丁烯二酸酶的筛选和生物催化工艺”; 

2009年-2012年, 天津市科技计划项目“(S)-3-异丁基-4-氨基丁酸(普瑞巴林)的生物催化合成”;

2008年-2011年, 中国科学院知识创新工程重要方向项目“新型酶催化剂的开发及其在有机合成中的应用研究”; 国家科技支撑项目“生物技术产品中试与规模化生产配套技术与工艺的研究与示范”之子课题;

2005年-2006年, National Institute of Health (Grant No 2R44GM064887-02A1 and 5R44GM064887-03, Total funding