- 黄芳
- 研究员

黄芳,女,博士,研究员,博士生导师。 |
1963年1月出生于甘肃酒泉市。1984年和1989年在兰州大学获学士和硕士学位。2003年在瑞典Stockholm University获博士学位。1992-1994年,在英国Horticultural Research International(HRI)进行访问研究。2003-2004年在瑞典Stockholm University做Research Fellow。任中国科学院植物研究所光合作用功能蛋白质组学创新研究组组长。《植物学报》编委。近年来,在PNAS、Molecular & Cellular Proteomics、Journal of Proteome Research、Proteomics、FEBS Journal、Microbiology等国际学术刊物发表论文十余篇。培养具有硕士和博士学位的研究生6名、博士后1名;正在培养的博士和硕士研究生9名。 主要研究工作: 1. 蓝藻光合系统的生物发生机制与调控: 主要以光合模式蓝藻(如Synechocystis sp. PCC 6803)为材料,利用生物化学、分子遗传学和光谱学等手段,探讨光合膜系统及膜蛋白系统演化与功能调控的分子机理。 2.绿藻光合作用与光合产氢的生物发生机制与调控 主要以光合作用与光合产氢的模式绿藻(如Chlamydomonas reinhardtii)为材料,采用蛋白质组学和分子遗传学手段,探讨光合作用与光合产氢的代谢网络调控规律并发现重要基因靶标,培育高光效产氢微藻株系。 3.光合微藻逆境适应分子机制 主要从细胞和亚细胞水平,研究光合微藻对逆境(如高光强、高盐碱等)的分子适应机制,发展微藻资源高效利用的途径和方法。 主持和参加的科研项目: [1] “莱茵衣藻e6突变体基因及其在光能转化中的作用”,国家自然科学基金,2013.1-2016.12(主持) [2] “衣藻乙酸代谢突变体ACM1基因克隆及其在光合产氢中的作用”,国家自然科学基金,2012.1-2015.12(主持) [3] “高效低成本直接太阳能化学及生物转化与利用的基础研究”,国家重点基础研究发展计划(973),2009.1-2013.12,第七课题(主持) [4] “提高作物养分与光合效率基因的克隆与功能验证”,国家转基因重大专项项目,2009.1-2011.12(主持) [5] “基于光合作用原理的微藻水光解制氢研究”,中国科学院重要方向项目,2009.1-2011.12(主持) [6] “蓝藻重要盐胁迫诱导表达蛋白生物学功能研究”,国家自然科学基金,2008.1-2010.12(主持) [7] “特殊生境植物资源的开发利用技术”,国家高技术研究发展计划(863)重点项目,2007.1-2010.12(参加) [8] “微藻光合放氢的生物学基础及调控途径”,中国科学院重要方向项目,2009.6-2010.12(参加) [9] “单细胞藻类质膜应答极端pH及其它逆境的蛋白组学研究”,国家自然科学基金,2007.1-2007.12(主持) [10] “涉入藻类产氢的蛋白质分子鉴定及其功能研究”,国家自然科学基金,2006.1-2008.12(主持) [11] “藻类产氢分子调控机理”,国家人事部留学人员择优重点项目,2007.1-2007.12(主持) 研究论文(注*为通讯作者): 2018 Li LY, Yang HM, Liu P, Ren WB, Wu XH, Huang F*. 2018. Combined impact of heat stress and phosphate deficiency on growth and photochemical activity of sheepgrass (Leymus chinensis). J. Plant Physiol., 231: 271-276. 2017 Zhao L, Cheng DM, Huang XH, Chen M, Dall’Osto L, Xing JL, Gao LY, Li LY, Wang YL, Bassi R, Peng LW, Wang YC, Rochaix J-D, Huang F*. 2017. A light harvesting complex-like protein in maintenance of photosynthetic components in Chlamydomonas. Plant Physiol., 174: 2419-2433. Xing J, Liu P, Zhao L, Huang F*. 2017. Deletion of CGLD1 impairs PSII and increases singlet oxygen tolerance of green alga Chlamydomonas reinhardtii. Front. Plant Sci., 8:2154 2016 Chen Mei, Zhang Jin, Zhao Lei, Xing Jiale, Peng Lianwei, Kuang Tingyun, Rochaix Jean-David, Huang Fang*. 2016. Loss of algal Proton Gradient Regulation 5 increases reactive oxygen species scavenging and H2 evolution. Journal of Integrative Plant Biology, 58(12): 943-946. Li Lingyu, Yang Haomeng, Ren Weibo, Liu Bei, Cheng Dongmei, Wu Xinghong, Gong Jirui, Peng Lianwei, Huang Fang*. 2016. Physiological and biochemical characterization of sheepgrass (Leymus chinensis) reveals insights into photosynthetic apparatus coping with low-phosphate stress conditions. Journal of Plant Biology, 59: 336-346. 2015 Li Li, Deng Dandan, Chen Xi, Wu Baomei, Hu Ke, Qiu Tianhang, Cui Suxia*, Huang Fang*. 2015. Expression of the moss PpLEA4-20 gene in rice enhances membrane protection and client proteins stability. Biochemical and Biophysical Research Communications, 460(2): 386-391. Chen Mei, Zhang Jin, Zhao Lei, Xing Jiale, Peng Lianwei, Kuang Tingyun, Rochaix Jean-David, Huang Fang*. 2016. Loss of algal proton gradient regulation 5 increases ros scavenging and h 2 evolution: h 2 production of chlamydomonas mutant lacking pgr5. Plant Physiology, 58(12), 943-946. 2014 Gao LY, Shen CT, Liao LB, Huang XH, Liu KH, Wang W, Guo LH, Jin WH, Huang F, Xu W, Wang YC. 2014. Functional proteomic discovery of Slr0110 as a central regulator of carbohydrate metabolism in Synechocystis sp. PCC 6803. Molecular & Cellular Proteomics, 13:204-219. Yang HM, Liao LB, Bo TT, Zhao L, Sun XW, Lu XF, Norling B, Huang F.* 2014. Slr0151 in Synechocystis sp. PCC 6803 is required for efficient repair of photosystem II under high-light condition. Journal of Integrative Plant Biology, 56:1136-1150. 2013 Zhao L, Chen M, Cheng DM, Yang HM, Sun YL, Zhou HY, Huang F.* 2013. Different B-type Methionine Sulfoxide Reductases in Chlamydomonas may Protect the Alga against High-light, Sulfur-depletion or Oxidative Stress. Journal of Integrative Plant Biology, 55: 1053-1067. 杨浩萌, 薛哲勇, 赵乐, 漆小泉, 黄芳*. 2013. 集胞藻slr1515基因提高转基因水稻植株剑叶长度及叶绿素含量. 植物学报, 48卷 5期 489-497页. 杨浩萌, 薛哲勇, 赵乐, 漆小泉, 黄芳*. 2013. 集胞藻sbtA基因具有提高水稻旗叶叶绿素含量及株高的功能. 植物生理学报, 9卷 4期 351-356页. Sun YL, Chen M, Yang HM, Zhang J, Kuang TY, Huang F.* 2013. Enhanced H2 photoproduction by down-regulation of ferredoxin-NADP+ reductase (FNR) in the green alga Chlamydomonas reinhardtii. International Journal of Hydrogen Energy. 38: 16029-16037. 2012 Li T, Yang HM, Cui SX, Suzuki I, Zhang LF, Li L, Bo TT, Wang J, Murata N, Huang F.* 2012. Proteomic study of the impact of Hik33 mutation in Synechocystissp. PCC 6803 under normal and salt stress conditions. Journal of Proteome Research, 1: 502-514 2011 Zhao L, Sun YL, Cui SX, Chen M, Yang HM, Liu HM, Chai TY, Huang F.* 2011. Cd-induced proteomic changes in hyperaccumulator Phytolacca americana. Chemosphere, 85: 56-66 2010及以前 Chen M, Zhao L, Sun YL, Cui SX, Zhang LF, Yang B, Wang J, Kuang TY, Huang F.* 2010. Proteomic analysis of hydrogen photoproduction in sulfur-deprived Chlamydomonas cells. Journal of Proteome Research, 9: 3854-3866. Zhang LF, Yang HM, Cui SX, Hu J, Wang J, Kuang TY, Norling B. Huang F.* 2009. Proteomic analysis of plasma membranes of cyanobacterium Synechocystis sp. strain PCC 6803 in response to high pH stress. Journal of Proteome Research, 8: 2892-2902. Cui SX, Hu J, Yang B, Shi L, Huang F, Tsai S, He YK. Zhang J. 2009. Proteomic characterization of Phragmites communis in ecotypes of swamp and desert dune. Proteomics, 9: 3950–3967. Zhou JX, Zhou J, Yang HM, Chen M. Huang F.* 2008. Characterization of two glutaminases from the filamentous cyanobacterium Anabaena sp. PCC 7120. FEMS Microbiology Letters, 289: 241-249. Zhou J, Zhou JX, Yang HM, Yan CS, Huang F.* 2008. Characterization of a sodium-regulated glutaminase from cyanobacterium Synechocystis sp. PCC 6803. Science in China Series C: Life Sciences, 51: 1066-1075. Rajalahti T, Huang F, Klement M, Pisareva T, Edman M, Sjostrom M, Wieslander A. Norling, B. 2007. Proteins in different Synechocystis compartments have distinguishing N-terminal features: a combined proteomics and multivariate sequence analysis. Journal of Proteome Research, 7: 2420-2434. Ran L, Huang F, Ekman M, Klint J, Bergman B. 2007. Proteomic analyses of the photoauto- and diazotrophically grown cyanobacterium Nostoc sp. PCC 73102. Microbiology-SGM, 153: 608–618. Huang F, Fulda S, Hagemann M, Norling B. 2006. Proteomic screening of salt-stress-induced changes in plasma membranes of Synechocystis sp. strain PCC 6803. Proteomics, 6: 910-920. Fulda S, Mikkat S, Huang F, Huckauf J, Marin K, Norling B, Hagemann M. 2006. Proteome analysis of salt stress response in the cyanobacterium Synechocystis sp. strains PCC 6803. Proteomics, 6: 2733-2745. Huang F, Hedman E, Funk C, Kieselbach T, Schroder PW, Norling B. 2004. Isolation of outer membrane of Synechocystis sp. PCC 6803 and its proteomic characterization. Molecular & Cellular Proteomics, 3: 586-595 Huang F, Parmryd I, Nilsson, F, Persson AL, Pakrasi HB, Andersson B, Norling B. 2004. Proteomics of Synechocystis sp strain PCC 6803 - Identification of plasma membrane proteins. Molecular & Cellular Proteomics, 1: 956-966 Zak E, Norling B, Maitra R, Huang F, Andersson B, Pakrasi HB. 2001. The initial steps of biogenesis of cyanobacterial photosystems occur in plasma membranes. Proceedings of the National Academy of Sciences of the United States of America, 98: 13443-13448. Fulda S, Huang F, Nilsson F, Hagemann M, Norling B. 2000. Proteomics of Synechocystis sp strain PCC 6803-Identification of periplasmic proteins in cells grown at low and high salt concentrations. European Journal of Biochemistry, 267: 5900-5907 Huang F, Zhang XP, Szigyarto C, Glaser E. 1998. Import and processing of nuclear-encoded mitochondrial proteins during development of pea plants. In Plant Mitochondria: From Gene to Function. (I.M. M?ller, P. Gardestr?m, K. Glimelius and E. Glaser, eds.). Backhuys Publishers, Leiden, The Netherlands, pp 267-271. Wen JQ, Huang F, Liang WS, Liang HG. 1997. Increase of HCN and beta-cyanoalanine synthase activity during ageing of potato tuber slices. Plant Science, 125: 147-151 文江祁, 黄芳, 梁厚果. 1997. 马铃薯块茎HCN的释放与CAS活性变化. 科学通报, 24: 8-20. 专利: [1] 黄芳,杨浩萌,赵乐,薛哲勇,周荷益,漆小泉; 一种集胞藻蛋白在改良水稻性状中的新用途; ZL201110270947.0; 2014.02.05 [2] 黄芳,杨浩萌,赵乐,薛哲勇,周荷益,漆小泉; 一种与二氧化碳浓缩机制相关的集胞藻蛋白的新用途; ZL2011102709517; 2014.05.14 [3] 黄芳,吴骊珠,王红艳,陈梅,孙永乐,赵磊,陈彬; 一种利用光合产氢微藻高效筛选放氢催化剂的方法; ZL201110100773.3; 2014.01.15 [4] 黄芳,赵磊; 提高衣藻产氢量的培养基及其制备方法; ZL201110230908.8; 2014.05.07 [5] 黄芳,杨浩萌,赵乐,薛哲勇,周荷益,漆小泉; 一种集胞藻蛋白的新用途; ZL201110258388.1; 2014.06.18 [6] 黄芳,赵磊,杨浩萌; 抗氧化及营养胁迫相关蛋白及其编码基因与应用; ZL201210479314.5; 2016.01.13 [7] 黄芳,赵磊,程冬梅; 具有光保护功能的蛋白及其编码基因与应用; ZL201210479164.8; 2016.01.13 [8] 黄芳,孙永乐,赵乐,杨浩萌,陈梅; 一种生物镉抗性相关蛋白及其编码基因; ZL20090923926.9; 2012.08.29 [9] 黄芳,陈梅,孙永乐; 产氢量提高的基因工程衣藻及其应用; ZL201110045287.6; 2012.10.10 [10] 黄芳,赵磊,陈梅,孙永乐; 一种高效筛选衣藻放氢突变体的方法; ZL201010233108.7; 2013.05.29 [11] 黄芳,赵磊,杨浩萌,陈梅; 用于扩增产氢微藻莱茵衣藻插入突变基因侧翼序列的引物及方法; ZL201110026604.X; 2013.09.04 [12] 黄芳,赵磊,陈梅,张锦; 一株超高放氢藻株及其应用; ZL201410014492.X; 2016.03.02 |