朱蕾蕾

中国科学院天津工业生物技术研究所

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  • 朱蕾蕾
  • 研究员
  • 天津空港经济区西七道32号

简历

1、教育经历

2001.09-2005.06,中国,江南大学,生物工程,学士学位

2005.09-2007.06,中国,江南大学,生物化工,硕士学位

2007.07-2009.06,德国,不来梅雅各布大学(Jacobs University Bremen),生物技术,博士学习

2009.07-2010.09,德国,亚琛工业大学(RWTH Aachen University),生物技术,博士学位

2、工作经历

2011.01-2016.07,德国,亚琛工业大学(RWTH Aachen University),研究小组组长(subgroup leader)

2016.08-至今,天津工业生物技术研究所,蛋白质定向进化研究组组长

研究方向:

发展并建立蛋白质工程中的突变文库及高通量筛选等关键技术和方法,并将其应用在重要工业/医药用酶/代谢工程中关键酶的特性改造中。在总结海量实验数据的基础上,从分子水平提炼影响酶特性的一般性规律,为提高工业、医药用酶、代谢工程中关键酶的应用提供必要的理论支撑。

代表论著:

1、论文

1. Cheng F., Yang J., Schwaneberg U., Zhu L. Rational surface engineering of an arginine deiminase (an antitumor enzyme) for increased PEGylation efficiency. Biotechnology and Bioengineering, 2019, DOI: 10.1002/bit.27011.

2.Anand D., Dhoke G. V., Kinzel J., Garakani T. M., Davari M. D., Bocola M., Zhu L. and Schwaneberg U.. Chiral separation of D/L-arginine with whole cells through an engineered FhuA nanochannel. 2019,55, 5431-5434.

3. Frauenkron?Machedjou V. J., Fulton A., Zhao J., Weber L., Jaege K.?E. , Schwaneberg U., and Zhu L. Exploring the full natural diversity of single amino acid exchange reveals that 40-60% of BSLA positions improve organic solvents resistance. Bioresource and Bioprocessing, 2018, 5:2.

4. Cheng F., Yang J., Bocola M., Schwaneberg U., Zhu L. Loop engineering reveals the importance of active-site-decorating loops and gating residue in substrate affinity modulation of arginine deiminase (an anti-tumor enzyme). Biochemical and Biophysical Research. 2018, 499(2), 233-238.

5. Markel U.*, Zhu L.*, Frauenkron-Machedjou V. Zhao J., J., Bocola M., Davari M. D., Jaeger K.E., Schwaneberg U. Are Directed Evolution Approaches Efficient in Exploring Nature’s Potential to Stabilize a Lipase in Organic Cosolvents? Catalysts, 2017, 7(5), 142.

6. Charan H., Glebe U., Anand D., Kinzel J., Zhu L., Bocola M., Garakani T. M., Schwaneberg U., B?ker A. Nano-thin walled micro-compartments from transmembrane protein-polymer conjugates. Soft Matter. 2017, 13(15):2866-2875.

7. Charan H., Kinzel J., Glebe U., Anand D., Garakani T. M., Zhu L., Bocola M., Schwaneberg U., B?ker A. Grafting PNIPAAm from β-barrel shaped transmembrane nanopores. Biomaterials. 2016;107:115-23.

8. Cheng F.*, Zhu L.*, Schwaneberg U. Directed evolution 2.0: improving and deciphering enzyme properties. Chemical Communication, 2015, 51(48), 9760-9772. (共同第一作者)

9. Cheng F., Kardashliev T., Pitzler C., Shehzad A, Lue H., Bernhagen J, Zhu L.*, Schwaneberg U.* A competitive flow cytometry screening system for arginine-metabolizing enzyme in cancer treatment. ACS Synthetic Biology, 2015, 4(7):768-775. (共同通讯作者)

10. Machedjoua J., Fulton A., Zhu L., Ankera C., Bocola M., Jaeger K. and Schwaneberg U. Towards understanding of directed evolution: >52% amino acid positions contributed to ionic liquid resistance of Bacillus subtilis lipase A (BSLA). ChemBioChem, 2015, 16(6), 937-945.

11. Fulton A., Machedjoua J., Skoczinski P., Wilhelm S., Zhu L., Schwaneberg U. and Jaeger K. Exploring the protein stability landscape: Bacillus subtilis lipase A as a model for detergent tolerance. ChemBioChem, 2015, 16(6):930-6.

12. Zhu L., Cheng F., Piatkowski V. and Schwaneberg U. Protein engineering of PpADI for improved thermostability. ChemBioChem. 2014, 15(2), 276-83.

13. Cheng F., Zhu L.#, Lue H., Bernhagen J, Schwaneberg U.# Directed arginine deiminase evolution for efficient inhibition of arginine-auxotrophic melanomas. Applied Microbiology and Biotechnology, 2014, 99(3), 1237-1247. (共同通讯作者)

14. Rijn P., Tutus M., Kathrein C., Zhu L., Wessling M., Schwaneberg U., and B?ker A. Challenges and Advances in the Field of Self-Assembled Membranes, Chemical Society Reviews. 2013, 42(16):6578-6592.

15. Liu H., Zhu L., Bocola M., Chen N.,Spiess A. C. and Schwaneberg U. Directed laccase evolution for improved ionic liquid resistance. Green Chemistry, 2013, 15, 1348-1355.

16. Philippart F., Arlt M., Gotzen S., Tenne S. J., Bocola M., Chen H. H., Zhu L., Schwaneberg U. and Okuda J. A hybrid ring-opening metathesis polymerization catalyst based on engineered ?-barrel protein FhuA. Chemistry a Euopean Journal. 2013, 19(41), 13865–13871.

17. Ni Y., Liu Y., Schwaneberg U., Zhu L., Li N., Li L., Sun Z. Rapid evolution of arginine deiminase for improved anti-tumor activity. Applied Microbiology Biotechnology. 2011, 90(1):193-201.

18. Zhu L., Rajni V., Roccatano D., Ni Y., Sun Z., and Schwaneberg U. A potential antitumor drug (arginine deiminase) reengineered for efficient operation under physiological conditions. ChemBioChem. 2010, 11(16):2294-301.

19. Zhu L., Tee K., Roccatano D., Sonmez B, Ni Y., Sun Z., and Schwaneberg U. Directed evolution of an antitumor drug (arginine deiminase PpADI) for increased activity at physiological pH. ChemBioChem. 2010, 11, 691-697.

20. Ni Y., Li Z., Sun Z., Zheng P., Liu Y. Zhu L., Schwaneberg U. Expression of arginine deiminase from Pseudomonas plecoglossicida CGMCC2039 in Escherichia coli and its anti-tumor activity. Current Microbiology. 2009, 58(6):593-8.

21. Liu Y., Zheng P, Sun Z., Ni Y., Dong J. and Zhu L. Economical succinic acid production from cane molasses by Actinobacillus succinogenes. Bioresource Technology. 2008, 99(6): 1736-1742.

22. Zhao L., Sun Z., Zheng P., Zhu L. Biotransformation of isoeugenol to vanillin by a novel strain, Bacillus fusiformis. Biotechnology Letters. 2005, 27, 1505-1509.

2、专著

Zhu L., Arlt M., Liu H., Schwaneberg U. Chapter 3: Channel protein FhuA as a promising biomolecular scaffold for bioconjugates. Bio-Synthetic Hybrid Materials and Bionanoparticles: A Biological Chemical Approach Towards Material Science. Royal Society of Chemistry,ISBN 9781849738224,2015.

3、专利

[1] Schwaneberg U., Zhu L. Directed evolution of arginine deiminase for increased activity at physiological pH, 德国,EP2295560A1 (WO 2011029696 A1),2011-03-17。

[2] 刘宇鹏, 孙志浩, 朱蕾蕾, 郑璞。一种微生物发酵生产丁二酸的菌种和方法,中国,CN 100432215 C,2008-11-12。

承担科研项目情况:

中国科学院重点部署项目子课题

中国科学院STS项目子课题

中国科学院-诺和诺德项目