- 韩方普
- 研究员
韩方普，博士，研究员，博士生导师
东北师范大学遗传与细胞研究所获博士学位；1998-2001年在以色列 Weizmann 研究所做博士后，从事小麦多倍体基因组进化研究；2001-2004年在加拿大农业部做Visiting Fellow 和 Biologist，从事小麦抗赤霉病分子标记和种质创新及小麦多倍体基因组进化研究；2004-2008年在美国 University of Missouri-Columbia 从事玉米功能基因组及植物人工染色体研究。2009年入选中国科学院'百人计划'。韩方普研究组主要从事小麦和玉米功能基因组、小麦染色体工程育种及植物人工染色体研究。
主要研究领域
远缘杂交育种和多倍体基因组进化
重点研究多倍体作物小麦及小偃麦的形成过程及机制。高效地转移、鉴定和跟踪外缘基因，发掘具有重要育种价值的易位系和关键基因。揭示多倍体作物中基因组之间的互作与优势的分子机理；创制、鉴定和评价小片段易位系和近缘种全基因组渗入系；分离并详细研究来自野生物种的高产、优质、抗病虫和抗逆基因；培育高产稳产、优质高效、抗病和耐逆的作物新品种。
植物着丝粒的结构和功能
在玉米着丝粒功能研究领域：研究玉米染色体着丝粒功能“失活-激活”的表观遗传学调控机制。探讨DNA甲基化、组蛋白修饰以及小RNA与着丝粒功能的内在联系。
植物减数分裂
减数分裂过程中同源染色体的配对起始、重组、取向和分离的分子机理是国际上研究的热点。将以小麦和玉米的特殊突变体为材料来研究上述问题，分离减数分裂相关基因并阐明其功能。
植物人工染色体
将利用不同的方法构建植物人工染色体。构建和优化适合多基因或完整代谢途径遗传转化的转基因载体。
植物基因定点突变及定向重组
随着玉米基因组序列的完成，需要发展一种有效的方法来利用已知的序列信息进行定点突变和置换，避免位置效应而进行重要基因功能的鉴定。利用人工锌指蛋白核酸酶技术对小麦和玉米的基因进行定点突变和置换， 将对基因功能研究和分子设计育种提供新的方法。
发表论文：1. Su H, Liu Y, Liu Y, Birchler JA and Han F. 2018. The Behavior of the Maize B Chromosome and Centromere. Genes 9: 476.2. Han F, Lamb JC, McCaw ME, Gao Z, Zhang B, Swyers NC and Birchler JA. 2018. Meiotic Studies on Combinations of Chromosomes With Different Sized Centromeres in Maize. Front Plant Sci 9: 785.3. Feng C, Su H, Bai H, Wang R, Liu Y, Guo X, Liu C, Zhang J, Yuan J, Birchler JA and Han F. 2018. High-efficiency genome editing using a dmc1 promoter-controlled CRISPR/Cas9 system in maize. Plant Biotechnol J 16: 1848-1857.4. Birchler JA and Han F. 2018. Barbara McClintock's Unsolved Chromosomal Mysteries: Parallels to Common Rearrangements and Karyotype Evolution. Plant Cell30: 771-779.5. Yuan J, Shi Q, Guo X, Liu Y, Su H, Guo X, Lv Z and Han F. 2017. Site-specific transfer of chromosomal segments and genes in wheat engineered chromosomes. J Genet Genomics 44: 531-539.6. Liu Y, Su H, Liu Y, Zhang J, Dong Q, Birchler JA and Han F. 2017. Cohesion and centromere activity are required for phosphorylation of histone H3 in maize. Plant J 92: 1121-1131.7. Zhang J and Han F. 2017. Centromere pairing precedes meiotic chromosome pairing in plants. Sci China Life Sci 60: 1197-1202.8. Wang J, Liu Y, Su H, Guo X and Han F. 2017. Centromere structure and function analysis in wheat-rye translocation lines. Plant J 91: 199-207.9. Su H, Liu Y, Dong Q, Feng C, Zhang J, Liu Y, Birchler J and Han F. 2017. Dynamic location changes of Bub1-phosphorylated-H2AThr133 with CENH3 nucleosome in maize centromeric regions. New Phytol 214: 682-694.10. Su H, Liu Y, Liu Y, Lv Z, Xie S, Gao Z, Pang J, Wang X and Han F. 2016. Dynamic chromatin changes associated with de novo centromere formation in maize euchromatin. Plant J 88: 854-866.11. Guo X, Su H, Shi Q, Fu S, Wang J, Zhang X and Han F. 2016. De nove centromere formation and centromeric sequence expansion in wheat and its wide hybrids. PLoS Genet 12: e1005997.12. Feng C, Yuan J, Wang R, Liu Y, Birchler J and Han F. 2016. Efficient targeted genome modification in maize using CRISPR/Cas9 system. J Genet Genomics 43: 37-43.13. Liu Y, Su H, Pang J, Gao Z, Wang X, Birchler J and Han F. 2015. Sequential de novo centromere formation and inactivation on a chromosomal fragment in maize. Proc Natl Acad Sci U S A 112: 1263-1271.14. Feng C, Liu Y, Su H, Wang H, Birchler J and Han F. 2015. Recent advances in plant centromere biology. Sci China Life Sci 58: 240-245.15. Guo X, Shi Q, Wang J, Hou Y, Wang Y and Han F. 2015. Characterization and genome changes of new amphiploids from wheat wide hybridization. J Genet Genomics 42: 459-461.16. Guo X, Han F. 2014. Asymmetric epigenetic modification and elimination of rDNA sequences by polyploidization in wheat. Plant Cell 26: 1-18.17. Yuan J, Guo X, Hu J, Lv Z and Han F. 2014. Characterization of two CENH3 genes and their roles in wheat evolution. New Phytol 206: 839-851.18. Zhang J, Zhang B, Su H, Birchler J and Han F. 2014. Molecular mechanisms of homologous chromoso me pairing and segregation in plants. J Genet Genomics 41: 117-123.19. Zhang B, Dong Q, Su H, Birchler J and Han F. 2014. Histone phosphorylation: its role during cell cycle and centromere identity in plants. Cytogenet Genome Res 143: 144-149.20. Zhang J, Pawloski W and Han F. 2013. Centromere pairing in early meiotic prophase requires active centromeres and precedes installation of the synaptonemal complex in maize. Plant Cell 25: 3900-3909.21. Fu S, Lv Z, Gao Z, Wu H, Pang J, Zhang B, Dong Q, Guo X, Wang X, Birchler J and Han F. 2013. De novo centromere formation on a chromosome fragment in maize. Proc Natl Acad Sci U S A 110: 6033-6036.22. Zhang B, Lv Z, Pang J, Liu Y, Guo X, Fu S, Li J, Dong Q, Wu H, Gao Z, Wang X and Han F. 2013. A functional centromere after loss of centromeric and gain of ectopic sequences. Plant Cell 25: 1979-1989.23. Zhang H, Bian Y, Gou X, Zhu B, Xu C, Qi B, Li N, Rustgi S, Zhou H, Han F, Jiang J, Wettstein D and Liu B. 2013. Persistent whole-chromosome aneuploidy is generally associated with nascent allohexaploid wheat. Proc Natl Acad Sci U S A 110: 3447-3452.24. Fu S, Lv Z, Guo X, Zhang X and Han F. 2013. Alteration of terminal heterochromatin and chromosome rearrangements in derivatives of wheat-rye hybrids. J Genet Genomics 40: 413-420.25. Deng C, Bai L, Fu S, Yin W, Zhang Y, Chen Y, Wang R, Zhang X, Han F and Hu Z. 2013. Microdissection and Chromosome Painting of the Alien Chromosome in an Addition Line of Wheat - Thinopyrum intermedium. PLoS One 14: e72564.26. Birchler J and Han F. 2013. Centromere epigenetics in plants. J Genet Genomics 40: 201-204.27. Gao Z, Han F, Danilova T, Lamb J, Albert P and Birchler J. 2013. Labeling meiotic chromosomes in maize with fluorescence in situ hybridization. Methods Mol Biol 990:35-43.28. Masonbrink R, Fu S, Han F and Birchler J. 2013. Heritable loss of replication control of a minichromosome derived from the B chromosome of Maize. Genetics 193: 77-84.29. Dong Q and Han F. 2012. Phosphorylation of H2A is associated with centromere function and maintenance in meiosis. Plant J 71: 800-809.30. Yu C, Han F, Zhang J, Birchler J and Peterson T. 2012. A transgenic system for generation of transposon Ac/Ds-induced chromosome rearrangements in rice. Theor Appl Genet 125: 1449-1462.31. Qi B, Huang W, Zhu B, Zhong X, Guo J, Zhao N, Xu C, Zhang H, Pang J, Han F and Liu B. 2012. Global transgenerational gene expression dynamics in two newly synthesized allohexaploid wheat (Triticum aestivum) lines. BMC Biol 10: 3.32. Fu S, Lv Z, Qi B, Guo X, Li J, Liu B and Han F. 2012. Molecular cytogenetic characterization of wheat-Thinopyrum elongatum addition, substitution and translocation lines with a novel source of resistance to wheat Fusarium Head Blight. J Genet Genomics 39: 103-110.33. Fu S, Gao Z, Birchler J and Han F. 2012. Dicentric chromosome formation and epigenetics of centromere formation in plants. J Genet Genomics 39: 125-130.34. Gao Z, Fu S, Dong Q, Han F and Birchler J. 2011. Inactivation of a centromere during the formation of a translocation in maize. Chromosome Res 19: 755-761.35. Koo D, Han F, Birchler J and Jiang J. 2011. Distinct DNA methylation patterns associated with active and inactive centromeres of the maize B chromosome. Genome Res 21: 908-914.36. Birchler J, Gao Z, Shanma A, Presting G and Han F. 2011. Epigenetic aspects of centromere function in plants. Curr Opin in Plant Biol 14: 217–222.37. Yin W, Birchler J and Han F. 2011. Maize centromeres: where sequences meets epigenetics. Frontiers in Biology 6: 102-108.38. Zhao N, Xu L, Li M, Zhang H, Zhu B, Qi B, Xu C, Han F and Liu B. 2011.Chromosomal and genome wide molecular changes associated with initial stages of allohexaploidization in wheat can be transit and incidental. Genome 54: 692-699.39. Zhao N, Zhu B, Li M, Wang L, Xu L, Zhang H, Zheng S, Qi B, Han F and Liu B. 2011. Extensive and heritable epigenetic remodeling and genetic stability accompany allohexaploidization of wheat. Genetics 188: 499-510.40. Han F, Gao Z and Birchler J. 2009. Reactivation of an Inactive Centromere Reveals Epigenetic and Structural Components for Centromere Specification in Maize. Plant Cell 21: 1929-1939.41. Birchler J and Han F. 2009. Maize Centromeres: Structure, Function and Epigenetics. Annu Rev Genet 43: 287-303.42. Birchler J, Gao Z and Han F. 2009. Pairing in Plant: import is important. Proc Natl Acad Sci USA 106: 19751-19752.43. Wolfgruber T, Sharma A, Schneider K, Albert P, Koo D, Shi J, Gao Z, Han F, Lee H, Xu R, Allison J, Birchler J, Jiang J, Dawe K and Presting G. 2009. Maize centromere structure and evolution: sequence analysis of centromeres 2 and 5 reveals a major role for retrotransposons. PLoS Genetics 5: e1000743.44. Lamb J, Yu W, Han F and James A. Birchler. 2008. Plant Centromeres. Plant Genomes. Genome Dynamics. 4: pp. 95-107.45. Vega J, Yu W, Han F, Kato A, Peters E and Birchler J. 2008. Placement of Cre-lox site-specific recombination cassette in the maize genome by Agrobacterium-mediated gene transformation with a BIBAC vector system. Plant Mol Biol 66: 587-598.46. Birchler J, Yu W and Han F. 2008. Plant engineered minichromosomes and artificial chromosome platforms. Cytogenet Genome Res 120: 228-232.47. Birchler J, Gao Z and Han F. 2008. A tale of two centromeres? diversity of structure but conservation of function in plants and animals. Funct & Integr Genomics 9: 7-13.48. Lamb J, Meyer J, Corcoran B, Kato A, Han F and Birchler J. 2007. Distinct chromosomal distributions of highly repetitive sequences in maize. Chromosome Res 15: 33-49.49. Lamb J, Han F, Yu W and Birchler J. 2007. Plant chromosomes from end to end: telomeres, heterochromatin and centromeres. Curr Opin Plant Biol 10: 116-122.50. Han F, Gao Z, Yu W, and Birchler J. 2007. Minichromosome analysis of chromosome pairing, disjunction and cohesion in maize. Plant Cell 19: 3853-3863.51. Han F, Lamb J, Yu W, Gao Z and Birchler J. 2007. Centromere function and nondisjunction are independent components of the maize B chromosome accumulation mechanism. Plant Cell 19: 524-533.52. Yu W, Lamb J, Han F and Birchler J. 2007. Cytological visualization of DNA transposons and their transposition pattern in somatic cells of maize. Genetics 175: 31-39.53. Yu W, Han F, Vega J, Gao Z and Birchler J. 2007. Construction and behavior of engineered minichromosome in maize. Proc Natl Acad Sci U S A 104: 8924-8929.54. Yu W, Han F and Birchler J. 2007. Engineered minichromosomes in plant. Curr Opin Biotechnol 18: 425-431.55. Han F, Lamb J and Birchler J. 2006. High frequency of centromere inactivation resulting in stable dicentric chromosomes of maize. Proc Natl Acad Sci USA 103: 3238-3243.56. Yu W, Lamb J, Han F and Birchler J. 2006. Telomere-associated chromosomal truncation in maize. Proc Natl Acad Sci U S A 103: 17331-17336.57. Han F, Fedak G, Guo W and Liu B. 2005. Rapid and repeatable elimination of a parental genomic specific DNA (pGc1R-1a) in newly synthesized wheat allopolyploids. Genetics 170: 1239-1245.58. Han F, Fedak G, Ouellet T, Dan H and Somers D. 2005. Mapping of genes expressed in Fusarium graminearum-infected heads of wheat cultivar ‘Frontana’. Genome 48: 88-96.59. Han F, Liu B, Fedak G and Liu Z. 2004. Chromosomal variation, constitution of five partial amphiploids of wheat-Thinopyrum intermedium detected by GISH, seed storage protein marker and multicolor GISH. Theor Appl Genetics 109: 1070-1076.60. Han F, Liu Z, Tan M, Hao S, Fedak G and Liu B. 2004. Mobilized endogenous retrotransposon Tos17 of rice by alien DNA introgression transposes into genes and causes heritable alteration in structure and cytosine methylation status of a flanking genomic region. Hereditas141: 243-251.
