屠强

中国科学院遗传与发育生物学研究所

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  • 屠强
  • 研究员

屠强,博士,研究员,博士生导师

工作经历2014 - 至今 中国科学院遗传发育所,研究员 2013 - 2014 美国加州理工学院,Member of the Professional Staff (校级学术委员会批准) 2010 - 2013 美国加州理工学院,Senior Research Fellow (Voting Faculty) (校级学术委员会批准) 2004 - 2010 美国加州理工学院,博士后 2003 - 2004 中国科学院蛋白组分析中心,研究助理

学习经历2003 博士 中国科学院生化细胞所 2000 硕士 复旦大学1997 学士 安徽师范大学

研究兴趣 我们的研究兴趣是发育系统生物学,更具体地说,我们的研究是综合实验和计算手段,用系统生物学方法,研究发育生物学问题,以期理解指导发育的基因调控网络。

我们为什么要研究基因调控网络因为发育过程不是被一个基因而是被一系列基因控制着的;这些基因也不是孤立存在的,它们之间存在着复杂的因果关系。

如何构建一个发育基因调控网络

1. 调控基因的鉴定和描述(时空表达信息)
2干扰实验: 干扰基因B,测量基因A/B/C/D的变化
再分别干扰基因A/C/D
3.顺式元件分析
4.最终

我们使用的动物模型:青鳉鱼 青鳉鱼是一种原产东亚的小型卵生淡水硬骨鱼。培养温度为28℃ 时,其胚胎九天后孵化,两三个月内达到性成熟。成鱼大约3厘米长。青鳉鱼是温带鱼,能够在 4℃到40℃的范围内生存。它的基因组比较小(800Mb),只有斑马鱼的一半。数十年来,青鳉被用作环境毒理学、致癌研究的测试系统。

发表文章(部分)1.Barsi JC*, Tu Q*, Calestani C, Davidson EH. Genome-wide assessment of differential effector gene use in embryogenesis. Development. 2015; 142(22): 3892-3901. *contributed equally. PMID: 264170442.Barsi JC*, Tu Q*, Davidson EH. General approach for in vivo recovery of cell type-specific effector gene sets. Genome Res 2014; 24: 860-868. * contributed equally. PMID: 246047813. Tu Q, Cameron RA, Davidson EH. Quantitative developmental transcriptomes of the sea urchin Strongylocentrotus purpuratus. Dev Biol. 2014; 385(2):160-167. PMID: 242911474. Tu Q, Cameron RA, Worley KC, Gibbs RA, Davidson EH. Gene structure in the sea urchin Strongylocentrotus purpuratus based on transcriptome analysis. Genome Res. 2012; 22(10): 2079-2087. PMID: 227097955. Oliveri P*, Tu Q*, Davidson EH. Global regulatory logic for specification of an embryonic cell lineage.Proc Natl Acad Sci U S A. 2008; 105(16):5955-62. * contributed equally. PMID: 18413610o Cover Article, Feature Articleo Comment: Hood L. Gene regulatory networks and embryonic specification. Proc Natl Acad Sci USA. 2008; 105(16): 5951-5952. PMID: 18417452o Comment: F1000.com6.Tu Q, Brown CT, Davidson EH, Oliveri P. Sea urchin Forkhead gene family: Phylogeny and embryonic expression. Dev Biol. 2006; 300(1):49-62. PMID: 170815127. Howard-Ashby M, Materna SC, Brown CT, Tu Q, Oliveri P, Cameron RA, Davidson EH. High regulatory gene use in sea urchin embryogenesis: Implications for bilaterian development and evolution. Dev Biol. 2006; 300(1):27-34. PMID: 171011258.Samanta MP, Tongprasit W, Istrail S, Cameron RA, Tu Q, Davidson EH, Stolc V. The transcriptome of the sea urchin embryo. Science. 2006; 314(5801):960-2. PMID: 170956949.Sea Urchin Genome Sequencing Consortium. The Genome of the Sea Urchin Strongylocentrotus purpuratus. Science. 2006; 314(5801):941-52. PMID: 1709569110.Tu Q, Tang H, Ding D. MedBlast: searching articles related to a biological sequence. Bioinformatics. 2004; 20(1):75-7. PMID: 1469381111.Tu Q, Ding D. Detecting pathogenicity islands and anomalous gene clusters by iterative discriminant analysis. FEMS Microbiol Lett. 2003; 221(2):269-75. PMID: 1272593812.Tu Q, Yu L, Zhang P, Zhang M, Zhang H, Jiang J, Chen C, Zhao S. Cloning, characterization and mapping of the human ATP5E gene, identification of pseudogene ATP5EP1, and definition of the ATP5E motif. Biochem J. 2000; 347(Pt 1):17-21. PMID: 1072739613.Zheng L, Yu L, Tu Q, Zhang M, He H, Chen W, Gao J, Yu J, Wu Q, Zhao S. Cloning and mapping of human PKIB and PKIG, and comparison of tissue expression patterns of three members of the protein kinase inhibitor family, including PKIA. Biochem J. 2000; 349(Pt 2):403-7. PMID: 1088033714.Lang T, Yu L, Tu Q, Jiang J, Chen Z, Xin Y, Liu G, Zhao S. Molecular cloning, genomic organization, and mapping of PRKAG2, a heart abundant gamma2 subunit of 5’-AMP-activated protein kinase, to human chromosome 7q36. Genomics. 2000; 70(2):258-63. PMID: 1111235415.Tu Q. A developing of the plant taxonomy tool software. Journal of Anhui Normal University (Natural Science). 1997; 20(2):189-93.