娄晓敏 ( LOU Xiaomin )

2010年1月至今 中国科学院北京基因组研究所 副研究员 硕士生导师

2007年7月-2010年12月 中国科学院北京基因组研究所 助理研究员

微量蛋白质组学技术的发展及肿瘤标志物的发现和功能研究

1. 973计划，人体消化道蛋白质组表达谱，2014-2017，结题，子课题负责人；

2. 863计划，肿瘤蛋白质分子标志物的研究与开发，2012-2015，结题，子课题负责人；

3. 973计划，结肠癌及其相关巨噬细胞的分泌蛋白质组与肿瘤发展的相关性研究，2011-2015，结题，子课题负责人；

4. 973计划，肿瘤微环境分泌蛋白质与动物模型和临床样本的关联分析，2011-2015，结题，子课题负责人；

5. 国家自然基金，天冬氨酸组织蛋白酶 D 与细胞衰老的关系研究，2009-2011，结题，项目负责人；

6. 973计划，炎症反应信号通路对肿瘤转移的影响，2009-2013，结题，学术骨干。

1. Liu Z, Gao Y, Hao F, Lou X, Zhang X, Li Y, Wu D, Xiao T, Yang L, Li Q, Qiu X, Wang E. Secretomes are a potential source of molecular targets for cancer therapies and indicate that APOE is a candidate biomarker for lung adenocarcinoma metastasis. Molecular Biology Reports. 2014, 41(11):7507-23.

2. Zhu X, Zhang J, Sun H, Jiang C, Dong Y, Shan Q, Su S, Xie Y, Xu N, Lou X*, Liu S*. Ubiquitination of Inositol-requiring Enzyme 1 (IRE1) by the E3 Ligase CHIP Mediates the IRE1/TRAF2/JNK Pathway. Journal of Biological Chemistry. 2014, 289(44):30567-77.

3. Lou X, Kang B, Zhang J, Hao C, Tian X, Li W, Xu N, Lu Y, Liu S. MFAP3L activation promotes colorectal cancer cell invasion and metastasis. Biochimica et Biophysica Acta-Molecular Basis of Disease. 2014, 1842(9):1423-32.

4. Zhang J, Lou X, Shen H, Zellmer L, Sun Y, Xu N, Liao D. Isoforms of wild type proteins often appear as low molecular weight bands on SDS-PAGE. Biotechnology Journal. 2014, 9(8):1044-54.

5. Lou X*, Zhang J, Liu S, Xu N*, Liao D*. The other side of the coin: The tumor-suppressive aspect of oncogenes and the oncogenic aspect of tumor-suppressive genes, such as those along the CCND-CDK4/6-RB axis. Cell Cycle. 2014, 13(11):1677-1693.

6. Ping X, Sun B, Wang L, Xiao W, Yang X, Wang W, Adhikari S, Shi Y, Lv Y, Chen Y, Zhao X, Li A, Yang Y, Dahal U, Lou X, Liu X, Huang J, Yuan W, Zhu X, Cheng T, Zhao Y, Wang X, Rendtlew Danielsen J, Liu F, Yang Y. Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase. Cell Research. 2014, 24(2):177-89.

7. Liu Y, Ying W, Ren Z, Gu W, Zhang Y, Yan G, Yang P, Liu Y, Yin X, Chang C, Jiang J, Fan F, Zhang C, Xu P, Wang Q, Wen B, Lin L, Wang T, Du C, Zhong J, Wang T, He QY, Qian X*, Lou X*, Zhang G*, Zhong F*. Chromosome-8-Coded Proteome of Chinese Chromosome Proteome Data Set (CCPD) 2.0 with Partial Immunohistochemical Verifications. J Proteome Res. 2014, 13(1):126-36.

8. Wang Q, Wen B, Wang T, Xu Z, Yin X, Xu S, Ren Z, Hou G, Zhou R, Zhao H, Zi J, Zhang S, Gao H, Lou X, Sun H, Feng Q, Chang C, Qin P, Zhang C, Li N, Zhu Y, Gu W, Zhong J, Zhang G, Yang P, Yan G, Shen H, Liu X, Lu H, Zhong F, He QY, Xu P, Lin L, Liu S. The omics evidences: single nucleotide variants transmissions on Chromosome 20 in liver cancer cell lines. J Proteome Res. 2013,13(1):200-11.

9. Zhang S, Wen B, Zhou B, Yang L, Cha C, Xu S, Qiu X, Wang Q, Sun H, Lou X, Zi J, Zhang Y, Lin L, Liu S. Correction to Quantitative Analysis of the Human AKR Family Members in Cancer Cell Lines Using the mTRAQ/MRM Approach. J Proteome Res. 2013, 12(9):4255.

10. Sun H, Lou X, Shan Q, Zhang J, Zhu X, Zhang J, Wang Y, Xie Y, Xu N, Liu S. Proteolytic characteristics of cathepsin D related to the recognition and cleavage of its target proteins. PLoS One. 2013, 8(6):e65733.

11. Yang L, Zhang J, Zhang S, Dong W, Lou X, Liu S. Quantitative Evaluation of Aldo–keto Reductase Expression in Hepatocellular Carcinoma (HCC) Cell Lines. Genomics, Proteomics & Bioinformatics. 2013, 11:230-40.

12. Zhang S, Wen B, Zhou B, Yang L, Cha C, Xu S, Qiu X, Wang Q, Sun H, Lou X, Zi J, Zhang Y, Lin L, Liu S. Quantitative analysis of the human AKR family members in cancer cell lines using the mTRAQ/MRM approach. J Proteome Res. 2013, 12(5):2022-33.

13. Wang Q, Wen B, Yan G, Wei J, Xie L, Xu S, Jiang D, Wang T, Lin L, Zi J, Zhang J, Zhou R, Zhao H, Ren Z, Qu N, Lou X, Sun H, Du C, Chen C, Zhang S, Tan F, Xian Y, Gao Z, He M, Chen L, Zhao X, Xu P, Zhu Y, Yin X, Shen H, Zhang Y, Jiang J, Zhang C, Li L, Chang C, Ma J, Yan G, Yao J, Lu H, Ying W, Zhong F, He QY, Liu S. Qualitative and quantitative expression status of the human chromosome 20 genes in cancer tissues and the representative cell lines. J Proteome Res. 2013, 12(1):151-61.

14. Shan Q, Lou X, Xiao T, Zhang J, Sun H, Gao Y, Cheng S, Wu L, Xu N, Liu S. A cancer/testis antigen microarray to screen autoantibody biomarkers of non-small cell lung cancer. Cancer Lett. 2013, 328:160-7.

15. Zhang X, Wang Q, Lou X, Sun H, Roepstorff P, Liu S. An on-target desalting and concentration sample preparation protocol for MALDI-MS and MS/MS analysis. Methods Mol Biol. 2012;909:17-28.

16. Zhang J, Lou X, Yang S, He S, Yang L, Liu M, Zhu H, Shan Q, Su S, Zhan Q, Xu N, Liu S. BAG2 is a target of the c-Myc gene and is involved in cellular senescence via the p21(CIP1) pathway. Cancer Lett. 2012, 318:34-41.

17. 房亚哲, 刘津, 吴琳, 娄晓敏, 赵冠飞, 张洪涛, 陈文明, 王清涛. 用尿液蛋白质组技术筛选多发性骨髓瘤相关蛋白的初步研究. 中国实验诊断学，04期， pp 647-650， 2011。

18. Yan S, Zhou C, Lou X, Xiao Z, Zhu H, Wang Q, Wang Y, Lu N, He S, Zhan Q, Liu S, Xu N. PTTG overexpression promotes lymph node metastasis in human esophageal squamous cell carcinoma. Cancer Res. 2009, 69(8):3283-90.

19. Lou X, Xiao T, Zhao K, Wang H, Zheng H, Lin D, Lu Y, Gao Y, Cheng S, Liu S, Xu N. Cathepsin D is secreted from M-BE cells: its potential role as a biomarker of lung cancer. J Proteome Res. 2007 Mar;6(3):1083-92.